Experimental evidence has accumulated to suggest that biologically efficacious informational effects can be derived mimicking active compounds solely through electromagnetic distribution upon aqueous systems affecting biological systems. Empirically rigorous demonstrations of antimicrobial agent associated electromagnetic informational inhibition of MRSA, Entamoeba histolytica, Trichomonas vaginalis, Candida albicans and a host of other important and various reported effects have been evidenced, such as the electro-informational transfer of retinoic acid influencing human neuroblastoma cells and stem teratocarcinoma cells. Cell proliferation and differentiation effects from informationally affected fields interactive with aqueous systems are measured via microscopy, statistical analysis, reverse transcription polymerase chain reaction and other techniques. Information associated with chemical compounds affects biological aqueous systems, sans direct systemic exposure to the source molecule. This is a quantum effect, based on the interactivity between electromagnetic fields, and aqueous ordered coherence domains. The encoding of aqueous systems and tissue by photonic transfer and instantiation of information rather than via direct exposure to potentially toxic drugs and physical substances holds clear promise of creating inexpensive non-toxic medical treatments.
An attempt to wake up the medical community to accept research done in the last 100 years proving that electromagnetic energy can replace brutal chemotherapy. Photo taken by a professional photographer, of his own daughter being treated for Neuroblastoma. The power of the image encouraged Andy to share it with others in order to highlight the 'real' face of childhood cancer. She died. The average cost for such treatment is in the order of 500k+.
Saturday, December 31, 2016
BEMER Electromagnetic Field Therapy Reduces Cancer Cell Radioresistance by Enhanced ROS Formation and Induced DNA Damage
Abstract
Each year more than 450,000 Germans are expected to be diagnosed with cancer subsequently receiving standard multimodal therapies including surgery, chemotherapy and radiotherapy. On top, molecular-targeted agents are increasingly administered. Owing to intrinsic and acquired resistance to these therapeutic approaches, both the better molecular understanding of tumor biology and the consideration of alternative and complementary therapeutic support are warranted and open up broader and novel possibilities for therapy personalization. Particularly the latter is underpinned by the increasing utilization of non-invasive complementary and alternative medicine by the population. One investigated approach is the application of low-dose electromagnetic fields (EMF) to modulate cellular processes. A particular system is the BEMER therapy as a Physical Vascular Therapy for which a normalization of the microcirculation has been demonstrated by a low-frequency, pulsed EMF pattern. Open remains whether this EMF pattern impacts on cancer cell survival upon treatment with radiotherapy, chemotherapy and the molecular-targeted agent Cetuximab inhibiting the epidermal growth factor receptor. Using more physiological, three-dimensional, matrix-based cell culture models and cancer cell lines originating from lung, head and neck, colorectal and pancreas, we show significant changes in distinct intermediates of the glycolysis and tricarboxylic acid cycle pathways and enhanced cancer cell radiosensitization associated with increased DNA double strand break numbers and higher levels of reactive oxygen species upon BEMER treatment relative to controls. Intriguingly, exposure of cells to the BEMER EMF pattern failed to result in sensitization to chemotherapy and Cetuximab. Further studies are necessary to better understand the mechanisms underlying the cellular alterations induced by the BEMER EMF pattern and to clarify the application areas for human disease.
Thursday, December 22, 2016
Effects of alternating magnetic field (12 gauss) on transplanted neuroblastoma
Abstract
Tuesday, December 20, 2016
Selective susceptibility to nanosecond pulsed electric field (nsPEF) across different human cell types.
Abstract
Tumor
ablation by nanosecond pulsed electric fields (nsPEF) is an emerging
therapeutic modality. We compared nsPEF cytotoxicity for human cell lines of
cancerous (IMR-32, Hep G2, HT-1080, and HPAF-II) and non-cancerous origin (BJ
and MRC-5) under strictly controlled and identical conditions. Adherent cells were
uniformly treated by 300-ns PEF (0-2000 pulses, 1.8 kV/cm, 50 Hz) on
indium tin oxide-covered glass coverslips, using the same media and serum.
Cell survival plotted against the number of pulses displayed three distinct
regions (initial resistivity, logarithmic survival decline, and residual
resistivity) for all tested cell types, but with differences in LD50 spanning
as much as nearly 80-fold. The non-cancerous cells were less sensitive than
IMR-32 neuroblastoma cells but more vulnerable than the other cancers
tested. The cytotoxic efficiency showed no apparent correlation with cell or
nuclear size, cell morphology, metabolism level, or the extent of membrane
disruption by nsPEF. Increasing pulse duration to 9 µs (0.75 kV/cm,
5 Hz) produced a different selectivity pattern, suggesting that
manipulation of PEF parameters can, at least for certain cancers, overcome
their resistance to nsPEF ablation. Identifying mechanisms and cell markers of
differential nsPEF susceptibility will critically contribute to the proper
choice and outcome of nsPEF ablation therapies.
Relevant publication - Electron Spin Interactions in Chemistry and Biology
To understand how magnetic fields affect biological cells, consult this book:
Electron Spin Interactions in Chemistry and Biology
Gertz Likhtenshtein
Dept of Chemistry
Ben-Gurion University of the Negev
Beersheba
Israel
https://books.google.com/books?id=1CG8DAAAQBAJ&pg=PA204&lpg=PA204&dq=biological+cell+electron+spin+in+magnetic+field&source=bl&ots=VRkMgQFP8L&sig=z0qgBfowFz6iDS3zCjfjJ-j54Pk&hl=en&sa=X&ved=0ahUKEwjst9XcmoPRAhUo1oMKHWAJBfMQ6AEINjAE#v=onepage&q=biological%20cell%20electron%20spin%20in%20magnetic%20field&f=false
Electron Spin Interactions in Chemistry and Biology
Gertz Likhtenshtein
Dept of Chemistry
Ben-Gurion University of the Negev
Beersheba
Israel
https://books.google.com/books?id=1CG8DAAAQBAJ&pg=PA204&lpg=PA204&dq=biological+cell+electron+spin+in+magnetic+field&source=bl&ots=VRkMgQFP8L&sig=z0qgBfowFz6iDS3zCjfjJ-j54Pk&hl=en&sa=X&ved=0ahUKEwjst9XcmoPRAhUo1oMKHWAJBfMQ6AEINjAE#v=onepage&q=biological%20cell%20electron%20spin%20in%20magnetic%20field&f=false
Tuesday, December 13, 2016
Bioeffects of Static Magnetic Fields: Oxidative Stress, Genotoxic Effects, and Cancer Studies
Abstract
The interaction of static magnetic fields (SMFs) with living organisms is a rapidly growing field of investigation. The magnetic fields (MFs) effect observed with radical pair recombination is one of the well-known mechanisms by which MFs interact with biological systems. Exposure to SMF can increase the activity, concentration, and life time of paramagnetic free radicals, which might cause oxidative stress, genetic mutation, and/or apoptosis. Current evidence suggests that cell proliferation can be influenced by a treatment with both SMFs and anticancer drugs. It has been recently found that SMFs can enhance the anticancer effect of chemotherapeutic drugs; this may provide a new strategy for cancer therapy. This review focuses on our own data and other data from the literature of SMFs bioeffects. Three main areas of investigation have been covered: free radical generation and oxidative stress, apoptosis and genotoxicity, and cancer. After an introduction on SMF classification and medical applications, the basic phenomena to understand the bioeffects are described. The scientific literature is summarized, integrated, and critically analyzed with the help of authoritative reviews by recognized experts; international safety guidelines are also cited.
Synergistic inhibitory effect of static magnetic field and antitumor drugs on Hepa1-6 cells
Abstract
Wednesday, December 7, 2016
Exposure to Strong Static Magnetic Field Slows the Growth of Human Cancer Cells In Vitro
Reductions of 19.04 k 7.3296, 22.06 k 6.19%, and 40.68 ? 8.31 % were measured for
the melanoma, ovarian carcinoma, and lymphoma cell lines, respectively, vs. control groups not exposed
to the magnetic field. Multicycle flow cytometry revealed that the cell cycle was largely unaltered.
Pulsed-field electrophoresis analysis revealed no increase in DNA breaks related to magnetic
field exposure. In conclusion, prolonged exposure to a very strong magnetic field appeared to inhibit
the growth of threc human tumor cell lines in vitro. The mechanism underlying this effect has not, as
yet, been identified, although alteration of cell growth cycle and gross fragmentation of DNA have
been excluded as possible contributory factors. Future investigations of this phenomenon may have
a significant impact on the future understanding and treatment of cancer.
https://deepblue.lib.umich.edu/bitstream/handle/2027.42/38293/?sequence=1
https://deepblue.lib.umich.edu/bitstream/handle/2027.42/38293/?sequence=1
BIOLOGICAL EFFECTS OF PULSATING MAGNETIC FIELDS: ROLE OF SOLITONS
In this paper, we analyze biological effects produced by magnetic fields in order to elucidate the physical
mechanisms, which can produce them.
We show that there is a chierarchy of such mechanisms and that the mutual interplay between them can result in the synergetic outcome. In particular, we analyze the biological effects of magnetic fields on soliton mediated charge transport in the redox processes in living organisms.
Such solitons are described by nonlinear systems of equations and represent electrons that are self-trapped in alpha-helical polypeptides due to the moderately strong electron-lattice interaction. They represent a particular type of disssipativeless large polarons in low-dimensional systems.
We show that the effective mass of solitons in the is different from the mass of free electrons, and that there is a resonant effect of the magnetic fields on the dynamics of solitons, and, hence, on charge transport that accompanies photosynthesis and respiration.
These effects can result in non-thermal resonant effects of magnetic fields on redox processes in particular, and on the metabolism of the organism in general.
This can explain physical mechanisms of therapies based on applying magnetic fields.
https://arxiv.org/ftp/arxiv/papers/1411/1411.6576.pdf
We show that there is a chierarchy of such mechanisms and that the mutual interplay between them can result in the synergetic outcome. In particular, we analyze the biological effects of magnetic fields on soliton mediated charge transport in the redox processes in living organisms.
Such solitons are described by nonlinear systems of equations and represent electrons that are self-trapped in alpha-helical polypeptides due to the moderately strong electron-lattice interaction. They represent a particular type of disssipativeless large polarons in low-dimensional systems.
We show that the effective mass of solitons in the is different from the mass of free electrons, and that there is a resonant effect of the magnetic fields on the dynamics of solitons, and, hence, on charge transport that accompanies photosynthesis and respiration.
These effects can result in non-thermal resonant effects of magnetic fields on redox processes in particular, and on the metabolism of the organism in general.
This can explain physical mechanisms of therapies based on applying magnetic fields.
https://arxiv.org/ftp/arxiv/papers/1411/1411.6576.pdf
Experimental evidence for 60 Hz magnetic fields operating through the signal transduction cascade: Effects on calcium influx and c-MYC mRNA induction
Significantly, lymphocytes exposed to the combination of magnetic fields plus suboptimal Con-A responded with an approximate 3.0-fold increase in band intensity of c-MYC mRNA transcripts.
Importantly, transcripts for the housekeeping gene GAPDH were not influenced by mitogen or magnetic fields. We also observed that lymphocytes that failed to exhibit increased calcium influx in response to magnetic fields plus Con-A, also failed to exhibit an increase in total copies of c-MYC mRNA.
Thus, calcium influx and c-MYC mRNA expression, which are sequentially linked via the signal transduction cascade in contrast to GAPDH, were both increased by magnetic fields.
These findings support the above ST hypothesis and provide experimental evidence for a general biological framework for understanding magnetic field interactions with the cell through signal transduction.
In addition, these findings indicate that magnetic fields can act as a co-stimulus at suboptimal levels of mitogen; pronounced physiological changes in lymphocytes such as calcium influx and c-MYC mRNA induction were not triggered by a weak mitogenic signal unless accompanied by a magnetic field.
Magnetic fields, thus, have the ability to potentiate or amplify cell signaling.
http://www.sciencedirect.com/science/article/pii/001457939380699U
Importantly, transcripts for the housekeeping gene GAPDH were not influenced by mitogen or magnetic fields. We also observed that lymphocytes that failed to exhibit increased calcium influx in response to magnetic fields plus Con-A, also failed to exhibit an increase in total copies of c-MYC mRNA.
Thus, calcium influx and c-MYC mRNA expression, which are sequentially linked via the signal transduction cascade in contrast to GAPDH, were both increased by magnetic fields.
These findings support the above ST hypothesis and provide experimental evidence for a general biological framework for understanding magnetic field interactions with the cell through signal transduction.
In addition, these findings indicate that magnetic fields can act as a co-stimulus at suboptimal levels of mitogen; pronounced physiological changes in lymphocytes such as calcium influx and c-MYC mRNA induction were not triggered by a weak mitogenic signal unless accompanied by a magnetic field.
Magnetic fields, thus, have the ability to potentiate or amplify cell signaling.
http://www.sciencedirect.com/science/article/pii/001457939380699U
Specific region of the c-myc promoter is responsive to electric and magnetic fields
Abstract
The level of c-myc transcripts is increased in cells exposed to extremely low frequency (elf) electromagnetic (EM) fields at 60 Hz. The aim of the present experiments was to determine if regulatory regions upstream of the c-myc gene modulate the response to EM fields. DNA upstream of P1 of both mouse and human c-myc genes was transfected into cells as CAT constructs. The presence of DNA 5' to the human or mouse myc genes results in increased expression of CAT following 20 min exposures of cells to 60 Hz elf EM fields. Specific portions of the human upstream DNA were deleted and introduced into cells. The region responsive to EM fields is located between -353 and -1,257 relative to the P1 promoter.
Electromagnetic energy as a bridge between atomic and cellular levels in the genetics approach to cancer treatment.
Abstract
Investigation on the effect of static magnetic field up to 30 mT on viability percent, proliferation rate and IC50 of HeLa and fibroblast cells.
Abstract
Moderate intensity static magnetic fields affect mitotic spindles and increase the antitumor efficacy of 5-FU and Taxol.
Abstract
Tuesday, December 6, 2016
The paradigm of biologically closed electric circuits (BCEC) and the formation of an International Association (IABC) for BCEC systems.
Abstract
https://www.ncbi.nlm.nih.gov/pubmed/7531025
http://hemingway.softwarelivre
Friday, December 2, 2016
Targeted treatment of cancer with radiofrequency electromagnetic fields amplitude-modulated at tumor-specific frequencies
In the past century, there have been many attempts to treat cancer with low levels of electric and magnetic fields.
We have developed noninvasive biofeedback examination devices and techniques and discovered that patients with the same tumor type exhibit biofeedback responses to the same, precise frequencies.
Intrabuccal administration of 27.12 MHz radiofrequency (RF) electromagnetic fields (EMF), which are amplitude-modulated at tumor-specific frequencies, results in long-term objective responses in patients with cancer and is not associated with any significant adverse effects.
Intrabuccal administration allows for therapeutic delivery of very low and safe levels of EMF throughout the body as exemplified by responses observed in the femur, liver, adrenal glands, and lungs.
In vitro studies have demonstrated that tumor-specific frequencies identified in patients with various forms of cancer are capable of blocking the growth of tumor cells in a tissue- and tumor-specific fashion.
Current experimental evidence suggests that tumor-specific modulation frequencies regulate the expression of genes involved in migration and invasion and disrupt the mitotic spindle.
This novel targeted treatment approach is emerging as an appealing therapeutic option for patients with advanced cancer given its excellent tolerability.
Dissection of the molecular mechanisms accounting for the anti-cancer effects of tumor-specific modulation frequencies is likely to lead to the discovery of novel pathways in cancer.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3845545/
We have developed noninvasive biofeedback examination devices and techniques and discovered that patients with the same tumor type exhibit biofeedback responses to the same, precise frequencies.
Intrabuccal administration of 27.12 MHz radiofrequency (RF) electromagnetic fields (EMF), which are amplitude-modulated at tumor-specific frequencies, results in long-term objective responses in patients with cancer and is not associated with any significant adverse effects.
Intrabuccal administration allows for therapeutic delivery of very low and safe levels of EMF throughout the body as exemplified by responses observed in the femur, liver, adrenal glands, and lungs.
In vitro studies have demonstrated that tumor-specific frequencies identified in patients with various forms of cancer are capable of blocking the growth of tumor cells in a tissue- and tumor-specific fashion.
Current experimental evidence suggests that tumor-specific modulation frequencies regulate the expression of genes involved in migration and invasion and disrupt the mitotic spindle.
This novel targeted treatment approach is emerging as an appealing therapeutic option for patients with advanced cancer given its excellent tolerability.
Dissection of the molecular mechanisms accounting for the anti-cancer effects of tumor-specific modulation frequencies is likely to lead to the discovery of novel pathways in cancer.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3845545/
Extremely low frequency electromagnetic fields affect proliferation and mitochondrial activity of human cancer cell lines.
PURPOSE:
MATERIALS AND METHODS:
RESULTS:
CONCLUSION:
Study of Electromagnetic Fields on Cellular Systems
These results suggest that MF
produce quantitative alterations
in protein. The DNA gel results
do not show signifi cant variations
and there is no evidence of apoptosis
when a MF of 19 mT at 8 Hz
is used. This result coincide with
other studies performed on neuroblastoma
where a MF of 1 – 2 mT
was applied at the rate of 50 – 60
Hz and no apoptosis was present
(Pirozzoli et al., 2003).
In conclusion, this paper shows
that a MF of 19 mT at a rate of 8
Hz can produces alterations in the
cells. However more research is need
to fully conclude the effects of EMF.
http://www.acuedi.org/ddata/1580.pdf
http://www.acuedi.org/ddata/1580.pdf
Wednesday, November 30, 2016
How a High-Gradient Magnetic Field Could Affect Cell Life
The biological effects of high-gradient magnetic
fields (HGMFs) have steadily gained the increased attention of researchers from
different disciplines, such as cell biology, cell therapy, targeted stem cell
delivery and nanomedicine. We present a theoretical framework towards a
fundamental understanding of the effects of HGMFs on intracellular processes,
highlighting new directions for the study of living cell machinery: changing
the probability of ion-channel on/off switching events by membrane magneto-mechanical
stress, suppression of cell growth by magnetic pressure, magnetically induced
cell division and cell reprograming, and forced migration of membrane receptor
proteins. By deriving a generalized form for the Nernst equation, we find that
a relatively small magnetic field (approximately 1 T) with a large gradient (up
to 1 GT/m) can significantly change the membrane potential of the cell and thus
have a significant impact on not only the properties and biological
functionality of cells but also cell fate
http://www.nature.com/articles/srep37407
http://www.nature.com/articles/srep37407
Monday, November 28, 2016
Effect of low frequency magnetic fields on melanoma: tumor inhibition and immune modulation
Results: The growth of B16-F10 cells was inhibited after exposure to the LF-MF. The inhibition was related to
induction of cell cycle arrest and decomposition of chromatins. Moreover, the LF-MF prolonged the mouse survival
rate and inhibited the proliferation of B16-F10 in melanoma metastasis mice model. Furthermore, the LF-MF
modulated the immune response via regulation of immune cells and cytokine production. In addition, the number
of Treg cells was decreased in mice with the LF-MF exposure, while the numbers of T cells as well as dendritic cells
were significantly increased.
Get the PDF paper here
induction of cell cycle arrest and decomposition of chromatins. Moreover, the LF-MF prolonged the mouse survival
rate and inhibited the proliferation of B16-F10 in melanoma metastasis mice model. Furthermore, the LF-MF
modulated the immune response via regulation of immune cells and cytokine production. In addition, the number
of Treg cells was decreased in mice with the LF-MF exposure, while the numbers of T cells as well as dendritic cells
were significantly increased.
Get the PDF paper here
The Effect of Alternating Magnetic Field Exposure and Vitamin C on Cancer Cells
The purpose of our study was to examine
the anti-tumor effect of vitamin C combined
with magnetic field treatments. The
inhibitory effect of vitamin C in cancer cells
involves its interaction with several compounds:
glutathione (GSH), hydrogen peroxide
and the enzyme catalase.17,18 In the
blood, vitamin C is oxidized to
dehydroascorbate (DHA). DHA is easily
transported across cell membranes where it
is then reduced by GSH back to vitamin C.
Cancer cells have a high level of GSH compared
to normal cells. The higher level of
GSH for the same level of vitamin C produces
more hydrogen peroxide. In normal
cells, catalase inactivates hydrogen peroxide
by converting it to water and oxygen. Cancer
cells have a reduced (10 to 100 fold) intracellular
level of catalase. This results in
very high levels of hydrogen peroxide and
oxidative by-products in the cancer cell.18
Hydrogen peroxide is toxic and destroys the
cancer cells.
Get the PDF paper here
the anti-tumor effect of vitamin C combined
with magnetic field treatments. The
inhibitory effect of vitamin C in cancer cells
involves its interaction with several compounds:
glutathione (GSH), hydrogen peroxide
and the enzyme catalase.17,18 In the
blood, vitamin C is oxidized to
dehydroascorbate (DHA). DHA is easily
transported across cell membranes where it
is then reduced by GSH back to vitamin C.
Cancer cells have a high level of GSH compared
to normal cells. The higher level of
GSH for the same level of vitamin C produces
more hydrogen peroxide. In normal
cells, catalase inactivates hydrogen peroxide
by converting it to water and oxygen. Cancer
cells have a reduced (10 to 100 fold) intracellular
level of catalase. This results in
very high levels of hydrogen peroxide and
oxidative by-products in the cancer cell.18
Hydrogen peroxide is toxic and destroys the
cancer cells.
Get the PDF paper here
Bioeffects of Static Magnetic Fields: Oxidative Stress, Genotoxic Effects, and Cancer Studies
These studies provide valuable insight into the phenomenon
of biomagnetism and open new avenues for the
development of new medical applications. Further studies are
necessary to explore the mechanisms of the SMF action in
more detail.
Get the paper (PDF)
of biomagnetism and open new avenues for the
development of new medical applications. Further studies are
necessary to explore the mechanisms of the SMF action in
more detail.
Get the paper (PDF)
Sunday, November 27, 2016
Elimination of Frequency Modulated Field Suppression of Melanoma Cell by Simultaneous Exposure to a Patterned Associated With Memory in Mammals
Discussion:
Many other experimenters [1-4] as well as our research
group have shown that exposures of malignant cells for one or
more hours per day for five or so consecutive days results in
consistent suppression of growth rates. Normal cells have not
been affected. The optimal point duration that is the specific
time each number that generates a particular voltage and a
specific strength of magnet fieldoccurs, is 3 ms. This point
duration has been associated with the properties of protons
within space [12], the duratin and conecntration of the
proton movements within the hydronium ion and their
dynamics within proton channels [21].
Abstract:
Strategic whole body application of physiologically patterned,
weak magnetic fields has been evolving as a
third option to treat cancer. Unlike chemical therapies and
ionizing radiation physiologically-patterned magnetic
fields diminish the growth of only malignant cells but not
normal cells. In addition these fields provide some
analgesic relief. To discern if competing magnetic fields
might abolish the beneficial effects mouse melanoma
cells in culture were exposed for 1 hour per day for 5 days
to decelerating frequency modulated patterned magnetic
fields that are well known for their growth suppressive
effects mediated by T-Type calcium channels. Typical
suppression rates were obtained. Exposure to a second
pattern that evokes LTP (long term potentiation) in
hippocampal slices and is the primary physical correlate of
memory, produced minimal effects. However when the
effective daily frequency-modulated hourly exposures
were preceded by only 30 min of LTP patterned magnetic
fields the growth suppression effects were completely
abolished. These results indicate certain patterned fields
cancel the beneficial effects and that the processes that
underlie proliferation of malignant cells and memory
consolidation in nervous tissue may share characteristics.
Get PDF here:
https://drive.google.com/open?id=0B5VdJBEJYIIvdjVOX2ttNjBlRkE
Many other experimenters [1-4] as well as our research
group have shown that exposures of malignant cells for one or
more hours per day for five or so consecutive days results in
consistent suppression of growth rates. Normal cells have not
been affected. The optimal point duration that is the specific
time each number that generates a particular voltage and a
specific strength of magnet fieldoccurs, is 3 ms. This point
duration has been associated with the properties of protons
within space [12], the duratin and conecntration of the
proton movements within the hydronium ion and their
dynamics within proton channels [21].
Abstract:
Strategic whole body application of physiologically patterned,
weak magnetic fields has been evolving as a
third option to treat cancer. Unlike chemical therapies and
ionizing radiation physiologically-patterned magnetic
fields diminish the growth of only malignant cells but not
normal cells. In addition these fields provide some
analgesic relief. To discern if competing magnetic fields
might abolish the beneficial effects mouse melanoma
cells in culture were exposed for 1 hour per day for 5 days
to decelerating frequency modulated patterned magnetic
fields that are well known for their growth suppressive
effects mediated by T-Type calcium channels. Typical
suppression rates were obtained. Exposure to a second
pattern that evokes LTP (long term potentiation) in
hippocampal slices and is the primary physical correlate of
memory, produced minimal effects. However when the
effective daily frequency-modulated hourly exposures
were preceded by only 30 min of LTP patterned magnetic
fields the growth suppression effects were completely
abolished. These results indicate certain patterned fields
cancel the beneficial effects and that the processes that
underlie proliferation of malignant cells and memory
consolidation in nervous tissue may share characteristics.
Get PDF here:
https://drive.google.com/open?id=0B5VdJBEJYIIvdjVOX2ttNjBlRkE
Inhibition of Cancer Cell Growth by Exposure to a Specific Time-Varying Electromagnetic Field Involves T-Type Calcium Channels
Abstract
Electromagnetic field (EMF) exposures affect many biological systems. The reproducibility of these effects is related to the intensity, duration, frequency, and pattern of the EMF. We have shown that exposure to a specific time-varying EMF can inhibit the growth of malignant cells. Thomas-EMF is a low-intensity, frequency-modulated (25-6 Hz) EMF pattern. Daily, 1 h, exposures to Thomas-EMF inhibited the growth of malignant cell lines including B16-BL6, MDA-MB-231, MCF-7, and HeLa cells but did not affect the growth of non-malignant cells. Thomas-EMF also inhibited B16-BL6 cell proliferation in vivo. B16-BL6 cells implanted in syngeneic C57b mice and exposed daily to Thomas-EMF produced smaller tumours than in sham-treated controls. In vitro studies showed that exposure of malignant cells to Thomas-EMF for > 15 min promoted Ca2+ influx which could be blocked by inhibitors of voltage-gated T-type Ca2+ channels. Blocking Ca2+ uptake also blocked Thomas-EMF-dependent inhibition of cell proliferation. Exposure to Thomas-EMF delayed cell cycle progression and altered cyclin expression consistent with the decrease in cell proliferation. Non-malignant cells did not show any EMF-dependent changes in Ca2+ influx or cell growth. These data confirm that exposure to a specific EMF pattern can affect cellular processes and that exposure to Thomas-EMF may provide a potential anti-cancer therapy.
The effects of static magnetic fields on viability and apoptosis in normal and cancerous cells
Abstract
Introduction: The influence of static magnetic fields on living organisms has been the topic of considerable interest for many years. However, the exact mechanism of static magnetic fields’ action is still unclear. This research was performed to evaluate possible relationship between static magnetic fields and cancer treatment and also determine the possible effects of co-treatment with anticancer drugs in normal and cancerous cells.
Methods: The effects of 10 mT static magnetic field on cell death (sub-G1 and apoptosis/necrosis) were investigated using flow cytometric methods. This study was performed in the presence and absence of cisplatin as an anticancer agent in HeLa cell line as cancerous and Hu02 as normal cell type.
Results: Static magnetic fields’ exposure causes an increase in cell death in HeLa cell line (at both times) and Hu02 at 24 h after treatment. Moreover, co-treatment of these fields and cisplatin led to an enhancement in cell death at 24 h via necrosis, early and late apoptosis, and a decrease in cell death in the first 48 h in both cell types.
Conclusion: Despite the fact that 10 mT intensity of static magnetic field is not a high value, it was able to change cell function and structure, which in turn leads to change in apoptosis rate. The data presented in this literature, may indicate that static magnetic field can decrease the cell death; but more investigations are needed to find the exact related mechanisms.
Methods: The effects of 10 mT static magnetic field on cell death (sub-G1 and apoptosis/necrosis) were investigated using flow cytometric methods. This study was performed in the presence and absence of cisplatin as an anticancer agent in HeLa cell line as cancerous and Hu02 as normal cell type.
Results: Static magnetic fields’ exposure causes an increase in cell death in HeLa cell line (at both times) and Hu02 at 24 h after treatment. Moreover, co-treatment of these fields and cisplatin led to an enhancement in cell death at 24 h via necrosis, early and late apoptosis, and a decrease in cell death in the first 48 h in both cell types.
Conclusion: Despite the fact that 10 mT intensity of static magnetic field is not a high value, it was able to change cell function and structure, which in turn leads to change in apoptosis rate. The data presented in this literature, may indicate that static magnetic field can decrease the cell death; but more investigations are needed to find the exact related mechanisms.
Effect of Static Magnetic Field on Oxidant/Antioxidant Parameters in Cancerous and Noncancerous Human Gastric Tissues
Conclusions
SMF accelerates peroxidation reactions possibly by suppressing SOD and GSH-Px enzymes in the cancerous gastric tissue. This event caused by SMF might play part in the death of cancer cells, which may be a good supportive vehicle for the cancer therapy.
https://www.researchgate.net/publication/303696250_Effect_of_Static_Magnetic_Field_on_OxidantAntioxidant_Parameters_in_Cancerous_and_Noncancerous_Human_Gastric_Tissues
SMF accelerates peroxidation reactions possibly by suppressing SOD and GSH-Px enzymes in the cancerous gastric tissue. This event caused by SMF might play part in the death of cancer cells, which may be a good supportive vehicle for the cancer therapy.
https://www.researchgate.net/publication/303696250_Effect_of_Static_Magnetic_Field_on_OxidantAntioxidant_Parameters_in_Cancerous_and_Noncancerous_Human_Gastric_Tissues
Effect of Magnetic Fields on Tumor Growth and Viability
Abstract
Breast cancer is the most common nonskin cancer and is the second leading cause of cancer-related deaths in women. Most methods of intervention involve combinations of surgery, chemotherapy, and ionizing radiation. Both chemotherapy and ionizing radiation can be effective against many types of cancer, but they also harm normal tissues. The use of nonionizing, magnetic fields has shown early promise in a number of in vitro and animal studies. Our study tested the effect of varying durations of magnetic exposure on tumor growth and viability in mice injected with breast cancer cells. Cancer cells were labeled through stable expression of firefly luciferase for monitoring of tumor growth and progression by using an in vivo imaging system. We hypothesized that magnetic field exposure would influence tumor growth and progression. Our results showed that exposure of the mice to magnetic fields for 360 min daily for as long as 4 wk suppressed tumor growth. Our study is unique in that it uses an in vivo imaging system to monitor the growth and progression of tumors in real time in individual mice. Our findings support further exploration of the potential of magnetic fields in cancer therapeutics, either as adjunct or primary therapy.
Electric and Electro-Magnetic Fields & Cancer Treatment
This is a fascinating field in cancer treatment. While various electro-magnetic-wave-based solutions have been proposed during the past century to treat cancer, only recently this treatment strategy started to gain momentum as both academia and the industry started to recognize the value. In this context, field based solutions for cancer treatment have even been approved recently by FDA to treat specific types of tumors.
http://www.cancertreatmentsresearch.com/?p=865
http://www.cancertreatmentsresearch.com/?p=865
Decline of cell viability and mitochondrial activity in mouse skeletal muscle cell in a hypomagnetic field
Abstract
http://onlinelibrary.wiley.com/doi/10.1002/bem.21968/abstract
Inhibition of Cancer Cell Growth by Exposure to a Specific Time-Varying Electromagnetic Field Involves T-Type Calcium Channels
Abstract
Electromagnetic field (EMF) exposures affect many biological systems. The reproducibility of these effects is related to the intensity, duration, frequency, and pattern of the EMF. We have shown that exposure to a specific time-varying EMF can inhibit the growth of malignant cells. Thomas-EMF is a low-intensity, frequency-modulated (25-6 Hz) EMF pattern. Daily, 1 h, exposures to Thomas-EMF inhibited the growth of malignant cell lines including B16-BL6, MDA-MB-231, MCF-7, and HeLa cells but did not affect the growth of non-malignant cells. Thomas-EMF also inhibited B16-BL6 cell proliferation in vivo. B16-BL6 cells implanted in syngeneic C57b mice and exposed daily to Thomas-EMF produced smaller tumours than in sham-treated controls. In vitro studies showed that exposure of malignant cells to Thomas-EMF for > 15 min promoted Ca2+ influx which could be blocked by inhibitors of voltage-gated T-type Ca2+ channels. Blocking Ca2+ uptake also blocked Thomas-EMF-dependent inhibition of cell proliferation. Exposure to Thomas-EMF delayed cell cycle progression and altered cyclin expression consistent with the decrease in cell proliferation. Non-malignant cells did not show any EMF-dependent changes in Ca2+ influx or cell growth. These data confirm that exposure to a specific EMF pattern can affect cellular processes and that exposure to Thomas-EMF may provide a potential anti-cancer therapy.http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0124136
Targeted treatment of cancer with radiofrequency electromagnetic fields amplitude-modulated at tumor-specific frequencies
In summary, our clinical results provide strong evidence that the intrabuccal administration of RF EMF amplitude-modulated at tumor-specific frequencies is safe and well-tolerated and may lead to long lasting therapeutic responses in patients with advanced cancer. Our in vitro experiments demonstrate that cancer cell proliferation can be targeted using tumor-specific modulation frequencies, which were identified in patients diagnosed with cancer. Tumor-specific modulation frequencies block the growth of cancer cells, modify gene expression, and disrupt the mitotic spindle (Figure 6). Studies are underway to dissect the biophysical mechanism leading cancer cells to respond to specific modulation frequencies identified in patients with a corresponding diagnosis of cancer but not to randomly selected or tumor-specific frequencies identified in other tumor types. Elucidation of this mechanism of action is likely to unveil novel pathways and targets.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3845545/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3845545/
Therapeutic Electromagnetic Field (TEMF) and gamma irradiation on human breast cancer xenograft growth, angiogenesis and metastasis
Conclusion
TEMF therapy provided a safe means for retarding tumor vascularization, growth and metastasis.
Electromagnetic effects-From cell biology to medicine
Abstract
In this review we compile and discuss the published plethora of cell biological effects which are ascribed to electric fields (EF), magnetic fields (MF) and electromagnetic fields (EMF). In recent years, a change in paradigm took place concerning the endogenously produced static EF of cells and tissues. Here, modern molecular biology could link the action of ion transporters and ion channels to the "electric" action of cells and tissues. Also, sensing of these mainly EF could be demonstrated in studies of cell migration and wound healing. The triggers exerted by ion concentrations and concomitant electric field gradients have been traced along signaling cascades till gene expression changes in the nucleus. Far more enigmatic is the way of action of static MF which come in most cases from outside (e.g. earth magnetic field). All systems in an organism from the molecular to the organ level are more or less in motion. Thus, in living tissue we mostly find alternating fields as well as combination of EF and MF normally in the range of extremely low-frequency EMF. Because a bewildering array of model systems and clinical devices exits in the EMF field we concentrate on cell biological findings and look for basic principles in the EF, MF and EMF action. As an outlook for future research topics, this review tries to link areas of EF, MF and EMF research to thermodynamics and quantum physics, approaches that will produce novel insights into cell biology
Magnetic Fields Encourage Cellular Reprogramming
http://cen.acs.org/articles/92/web/2014/10/Magnetic-Fields-Encourage-Cellular-Reprogramming.html
Biologists have been building up evidence that magnetic fields affect living things, says Michael Levin, director of Tufts University’s Center for Regenerative & Developmental Biology, who was not involved in the new study. For example, plants and amphibian embryos develop abnormally when shielded from Earth’s geomagnetic field. And there’s some clinical evidence that particular electromagnetic frequencies promote bone fracture healing and wound repair
Biologists have been building up evidence that magnetic fields affect living things, says Michael Levin, director of Tufts University’s Center for Regenerative & Developmental Biology, who was not involved in the new study. For example, plants and amphibian embryos develop abnormally when shielded from Earth’s geomagnetic field. And there’s some clinical evidence that particular electromagnetic frequencies promote bone fracture healing and wound repair
Moderate intensity static magnetic fields affect mitotic spindles and increase the antitumor efficacy of 5-FU and Taxol.
Abstract
Bioeffects of Static Magnetic Fields: Oxidative Stress, Genotoxic Effects, and Cancer Studies
5. Summary and Conclusions
In recent years, an abundance of research papers, review papers, and books has been published describing the possible physical and biological interactions of magnetic fields.Considering these articles comprehensively, the conclusions are as follows: the primary cause of changes in cells after incubation in external SMF is disruption of free radical metabolism and elevation of their concentration. Such disruption causes oxidative stress and, as a result, damages ion channels, leading to changes in cell morphology and expression of different genes and proteins and also changes in apoptosis and proliferation. Moreover, based on available data, it was concluded that exposure to SMFs alone has no or extremely small effects on cell growth and genetic toxicity regardless of the magnetic density. However, in combination with other external factors such as ionizing radiation and some chemicals such as cadmium, there is evidence strongly suggesting that an SMF modifies their effects. Effects of SMFs on apoptosis are a potentially interesting phenomenon. However, these effects often depended on a cell type and were not found in various types of cells. Many researchers have observed the effects of SMFs on tumor cells, particularly the inhibiting effects. In order to reduce the toxicity and resistance of single anticancer drugs, a variety of unified treatments were required. The synergy of magnetic fields and anticancer drugs was one of the methods. It provides a new strategy for the effective treatment of cancer.
These studies provide valuable insight into the phenomenon of biomagnetism and open new avenues for the development of new medical applications. Further studies are necessary to explore the mechanisms of the SMF action in more detail.
https://www.hindawi.com/journals/bmri/2013/602987/
Friday, November 25, 2016
Reactive Oxygen Species and PEMF
Purpose
The redox milieu, together with reactive oxygen species (ROS) accumulation, may play a role in mediating some biological effects of extremely-low-frequency electromagnetic fields (ELF-EMF). Some of us have recently reported that a pulsed EMF (PEMF) improves the antioxidant response of a drug-sensitive human neuroblastoma SH-SY5Y cell line to pro-oxidants. Since drug resistance may affect cell sensitivity to redox-based treatments, we wanted to verify whether drug-resistant human neuroblastoma SK-N-BE(2) cells respond to a PEMF in a similar fashion.
Materials and methods
SK-N-BE(2) cells were exposed to repeated 2 mT, 75 Hz PEMF (15 min each, repeated 3 times over 5 days), and ROS production, Mn-dependent superoxide dismutase (MnSOD)-based antioxidant protection and viability were assessed after 10 min or 30 min 1 mM hydrogen peroxide. Sham controls were kept at the same time in identical cell culture incubators.
Results
The PEMF increased the MnSOD-based antioxidant protection and reduced the ROS production in response to a pro-oxidant challenge.
Conclusions
Our work might lay foundation for the development of non-invasive PEMF-based approaches aimed at elevating endogenous antioxidant properties in cellular or tissue models.
Effects of 50Hz Magnetic Field Exposure on Neuroblastoma Morphology
https://drive.google.com/file/d/0B5VdJBEJYIIvZENpNUpBdUc1Mzg/view?usp=sharing
""In our experiments the exposure for 5 days to 50Hz, 2mT magnetic field inhibits cell growth proliferation with a decrease of 30% in exposed cells respect to the control.""
""In our experiments the exposure for 5 days to 50Hz, 2mT magnetic field inhibits cell growth proliferation with a decrease of 30% in exposed cells respect to the control.""
Evidences of the static magnetic field influence on cellular systems
Evidences of the static magnetic field influence on cellular systems
The link to this paper is an analysis of publications on NIH PubMed that give proof that magnetic fields affect living cells, many of them with remarkable results for cancer.
Abstract
Efforts to elucidate the doubtful character of the static magnetic field (SMF) influence on living cells have been made, although the topic still faces controversies because confusing reports in the scientific literature. This study intended to collect the most relevant issues separated by different topics (relating the SMF to its action on cellular systems) and analyze how the many field intensities, cell types and exposure time would affect the cell or intracellular structures. The analysis was based in the search in online databases aiming to give a general view of how the data can show conformity. It is proposed that scientists have been searching for linearity in what is actually a well characterized nonlinear system and two outputs are considered: the high sensibility of the parameters to arise effects and also the complexity and particularity of each cellular system. It is possible to trigger effects from a SMF, however in a stochastic way and depending on the cell system.
Welcome to the Medical Anarchist!
Welcome to this blog. I work indirectly with researchers trying to find a cure for cancer. When hired a few years ago, I had been collecting magnets because they fascinated me, and I wanted to do some independent research with them. While at work, I researched magnets and cancer and found troves and troves of scientific publications, indicating that electromagnetic fields can cure cancer or at least make cancer cells more permeable to chemotherapy drugs. I deluged our main researcher with tons of these papers, admonishing him that we need to bring this technology to the mainstream medical community. For all intents and purposes, my research was ignored.
While going to work one day, my right knee blew out, I could barely walk. I gave it a few days and it got worse and worse. I had surgery on my other knee, so realized I probably needed surgery. I was going to call the next day to arrange it, but was not looking forward to the cost and impact to my job. As I was researching medical applications of magnets, I figured, what the heck, and took a magnet my friend extracted from a computer hard drive and attached it to my knee brace that night. Lo and behold, in the morning it was 90 percent better! Months later, it is good as new. At that point I was convinced of the medical applications of magnets.
Then I saw this article where a father documented his daughter dying from neuroblastoma:
http://www.foxnews.com/health/2016/11/02/father-shares-photo-daughter-near-end-cancer-battle-to-raise-awareness.html
The last paragraph in the article was the same sentiment I had while wondering why our scientist don't pursue electromagnetism as a cure:
“Please I beg of you, as a heartbroken father, it is too late for my daughter, but childhood cancer needs to be cured,” Andy wrote. “No family should have to go through this hell.”
The goal of this blog is to share with you articles and papers that give evidence to the fact that electromagnetic fields affect living cells, and should be seriously explored as a cancer treatment, NOW. I recently read an article where a world class researcher into child neuroblastoma stated something along the lines that "we need to explore other options, because we are giving these children the most toxic poisons known to mankind, there is something wrong with that" - I contacted her about my research and received no reply.
While going to work one day, my right knee blew out, I could barely walk. I gave it a few days and it got worse and worse. I had surgery on my other knee, so realized I probably needed surgery. I was going to call the next day to arrange it, but was not looking forward to the cost and impact to my job. As I was researching medical applications of magnets, I figured, what the heck, and took a magnet my friend extracted from a computer hard drive and attached it to my knee brace that night. Lo and behold, in the morning it was 90 percent better! Months later, it is good as new. At that point I was convinced of the medical applications of magnets.
Then I saw this article where a father documented his daughter dying from neuroblastoma:
http://www.foxnews.com/health/2016/11/02/father-shares-photo-daughter-near-end-cancer-battle-to-raise-awareness.html
The last paragraph in the article was the same sentiment I had while wondering why our scientist don't pursue electromagnetism as a cure:
“Please I beg of you, as a heartbroken father, it is too late for my daughter, but childhood cancer needs to be cured,” Andy wrote. “No family should have to go through this hell.”
The goal of this blog is to share with you articles and papers that give evidence to the fact that electromagnetic fields affect living cells, and should be seriously explored as a cancer treatment, NOW. I recently read an article where a world class researcher into child neuroblastoma stated something along the lines that "we need to explore other options, because we are giving these children the most toxic poisons known to mankind, there is something wrong with that" - I contacted her about my research and received no reply.
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