Wednesday, February 28, 2018

Disregard, unless you are a Researcher

This is theory which supports all the other posts, most visitors should disregard this informative publication, as it is high level theory:

The Biotic Pattern of Prime Numbers Supports the Bios Theory of Creative Evolution from Radiation to Complexity
http://necsi.edu/events/iccs7/papers/3114216e0aebd76eb51ebe74680c.pdf

Sunday, February 25, 2018

Towards non-invasive cancer diagnostics and treatment based on electromagnetic fields, optomechanics and microtubules

In this paper, we discuss biological effects of electromagnetic (EM) fields in the context of cancer biology. In particular, we review the nanomechanical properties of microtubules (MTs), the latter being one of the most successful targets for cancer therapy. We propose an investigation on the coupling of electromagnetic radiation to mechanical vibrations of MTs as an important basis for biological and medical applications. In our opinion optomechanical methods can accurately monitor and control the mechanical properties of isolated MTs in a liquid environment. Consequently, studying nanomechanical properties of MTs may give useful information for future applications to diagnostic and therapeutic technologies involving non-invasive externally applied physical fields. For example, electromagnetic fields or high intensity ultrasound can be used therapeutically avoiding harmful side effects of chemotherapeutic agents or classical radiation therapy.
https://www.researchgate.net/publication/319327215_Towards_non-invasive_cancer_diagnostics_and_treatment_based_on_electromagnetic_fields_optomechanics_and_microtubules

Friday, February 16, 2018

Electric fields and biological cells: numerical insight into possible interaction mechanisms

Exposure of cells and tissues to electric fields can lead to a variety of physiological changes. These changes are caused by the interactions between the incident field and biological, chemical and electric (magnetic) processes. Evaluating whether the physiological changes are beneficial or harmful to the functionality of cells and tissues requires understanding the nature of the interactions, which can be associated with either thermal or non-thermal (bio)-effects. Although a considerable amount of research worldwide has shed light on these effects and their influence in the biological response, much more is left to say about the possible interaction mechanisms and the underlying biological processes modified by the incident field. In fact, the adequate conditions of exposure (dosimetry) needed for cells and tissues to trigger a certain positive response and avoid possible damage are still a matter of debate. Moreover, concerns coming from social sectors about the hazards of the exposure have motivated multiple studies that have yielded controversial results. In view of the lack of knowledge on the biological cues suitable for explaining the effects, and in the light of the social interest in clarifying possible risks derived from the exposure, the aim of this thesis is to identify and assess possible interaction mechanisms between an incident electric field and biological cells and tissues. These mechanisms are elucidated upon determination of the electrical response of biological cells exposed to electric fields, which is obtained by means of the numerical implementation and solution of mathematical models formulated from a biophysics standpoint which combines the fundamentals of biological cells and tissues with the electromagnetic theory. In a first group of models, a numerical approach suitable to model multiple cells based on the quasi-static approximation and the equivalent dipole moment of a dielectric sphere is presented.
https://pure.tue.nl/ws/files/10243383/20151217_CO_Vanegas.pdf

Sunday, February 4, 2018

Bioeffects of Static Magnetic Fields: Oxidative Stress, Genotoxic Effects, and Cancer Studies

Abstract

The interaction of static magnetic fields (SMFs) with living organisms is a rapidly growing field of investigation. The magnetic fields (MFs) effect observed with radical pair recombination is one of the well-known mechanisms by which MFs interact with biological systems. Exposure to SMF can increase the activity, concentration, and life time of paramagnetic free radicals, which might cause oxidative stress, genetic mutation, and/or apoptosis. Current evidence suggests that cell proliferation can be influenced by a treatment with both SMFs and anticancer drugs. It has been recently found that SMFs can enhance the anticancer effect of chemotherapeutic drugs; this may provide a new strategy for cancer therapy. This review focuses on our own data and other data from the literature of SMFs bioeffects. Three main areas of investigation have been covered: free radical generation and oxidative stress, apoptosis and genotoxicity, and cancer. After an introduction on SMF classification and medical applications, the basic phenomena to understand the bioeffects are described. The scientific literature is summarized, integrated, and critically analyzed with the help of authoritative reviews by recognized experts; international safety guidelines are also cited.

Low intensity and frequency pulsed electromagnetic fields selectively impair breast cancer cell viability.

Abstract

INTRODUCTION:

A common drawback of many anticancer therapies is non-specificity in action of killing. We investigated the potential of ultra-low intensity and frequency pulsed electromagnetic fields (PEMFs) to kill breast cancer cells. Our criteria to accept this technology as a potentially valid therapeutic approach were: 1) cytotoxicity to breast cancer cells and; 2) that the designed fields proved innocuous to healthy cell classes that would be exposed to the PEMFs during clinical treatment.

METHODS:

MCF7 breast cancer cells and their normal counterparts, MCF10 cells, were exposed to PEMFs and cytotoxic indices measured in order to design PEMF paradigms that best kill breast cancer cells. The PEMF parameters tested were: 1) frequencies ranging from 20 to 50 Hz; 2) intensities ranging from 2 mT to 5 mT and; 3) exposure durations ranging from 30 to 90 minutes per day for up to three days to determine the optimum parameters for selective cancer cell killing.

RESULTS:

We observed a discrete window of vulnerability of MCF7 cells to PEMFs of 20 Hz frequency, 3 mT magnitude and exposure duration of 60 minutes per day. The cell damage accrued in response to PEMFs increased with time and gained significance after three days of consecutive daily exposure. By contrast, the PEMFs parameters determined to be most cytotoxic to breast cancer MCF-7 cells were not damaging to normal MCF-10 cells.

CONCLUSION:

Based on our data it appears that PEMF-based anticancer strategies may represent a new therapeutic approach to treat breast cancer without affecting normal tissues in a manner that is non-invasive and can be potentially combined with existing anti-cancer treatments.

Effect of Magnetic Field on the Biological Clock through the Radical Pair Mechanism

The Nobel prize for medicine was awarded last year based on this subject:

Abstract—There is an ongoing controversy in the literature related to the biological effects of weak, low frequency electromagnetic fields. The physical arguments and interpretation of the experimental evidence are inconsistent, where some physical arguments and experimental demonstrations tend to reject the likelihood of any effect of the fields at extremely low level. The problem arises of explaining, how the low-energy influences of weak magnetic fields can compete with the thermal and electrical noise of cells at normal temperature using the theoretical studies. The magnetoreception in animals involve radical pair mechanism. The same mechanism has been shown to be involved in the circadian rhythm synchronization in mammals. These reactions can be influenced by the weak magnetic fields. Hence, it is postulated the biological clock can be affected by weak magnetic fields and these disruptions to the rhythm can cause adverse biological effects. In this paper, likelihood of altering the biological clock via the radical pair mechanism is analyzed to simplify these studies of controversy.
http://people.eng.unimelb.edu.au/malkah/Publications/2010_BiologicalClock.pdf

Experimental studies on ultralow frequency pulsed gradient magnetic field inducing apoptosis of cancer cell and inhibiting growth of cancer cell

The morphology characteristics of cell apoptosis of the malignant tumour cells in magnetic field-treated mouse was observed for the first time. The apoptotic cancer cell contracted, became rounder and divorced from adjacent cells; the heterochromatin condensed and coagulated together along the inner side of the nuclear membrane; the endoplasmic reticulums (ER) expanded and fused with the cellular membrane; many apoptotic bodies which were packed by the cellular membrane appeared and were devoured by some lymphocytes and plasma. Apoptosis of cancer cells was detected by terminal deoxynucleotidyl transferase mediatedin situ nick end labeling (TUNEL). It was found that the number of apoptosis cancer cells of the sample treated by the magnetic field is more than that of the control sample. The growth of malignant tumour in mice was inhibited and the ability of immune cell to dissolve cancer cells was improved by ultralow frequency (ULF) pulsed gradient magnetic field; the nuclei DNA contents decreased, indicating that magnetic field can block DNA replication and inhibit mitosis of cancer cells. It was suggested that magnetic field could inhibit the metabolism of cancer cell, lower its malignancy, and restrain its rapid and heteromorphic growth. Since ULF pulsed gradient magnetic field can induce apoptosis of cancer cells and inhibit the growth of malignant tumour, it could be used as a new method to treat cancer.
https://rd.springer.com/article/10.1360/02yc9004?no-access=true